Theory of multiple-detection size-exclusion chromatography of complex branched polymers
Résumé
Size exclusion chromatography (SEC) separates complex branched polymers by hydrodynamic volume, rather than by molecular weight or branching characteristics. Equations relating the response of different types of detectors are derived including band broadening, by defining a distribution function N'(M,Vh), the number of chains with molecular weight M and hydrodynamic volume Vh. While the true molecular weight distribution of complex polymers cannot be determined by SEC whatever detector is used, the formalism enables multiple detection SEC data to be processed to both analyze the polymer sample and reveal mechanistic information about the polymer synthesis. The formalism also shows how the true weight- and number-average molecular weight, Mw and Mn, can be obtained from correct processing of the hydrodynamic volume distributions.
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