Transcriptomic response to parasite infection in Nile tilapia (Oreochromis niloticus) depends on rearing density - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue BMC Genomics Année : 2018

Transcriptomic response to parasite infection in Nile tilapia (Oreochromis niloticus) depends on rearing density

Résumé

Background Captive animal populations, be it for food production or conservation programmes, are often maintained at densities far beyond those in natural environments, which can have profound effects on behaviour, immune and stress levels, and ultimately welfare. How such alterations impact transcriptional responses to pathogen infection is a ‘different kettle of fish’ and remains poorly understood. Here, we assessed survival and gene expression profiles of infected fish reared at two different densities to elucidate potential functional genomic mechanisms for density-related differences in disease susceptibility. Results Utilising a whole-transcriptome sequencing (RNAseq) approach, we demonstrate that rearing density in tilapia (Oreochromis niloticus) significantly impacts susceptibility to the oomycete Saprolegnia parasitica, via altered transcriptional infection responses. Tilapia held at low densities have increased expression of genes related to stress, likely due to increased aggressive interactions. When challenged with Saprolegnia, low-density fish exhibit altered expression of inflammatory gene responses and enhanced levels of adaptive immune gene suppression compared to fish reared at higher density, resulting in significantly increased mortality rates. In addition, Saprolegnia infection substantially perturbs expression of circadian clock genes, with fish reared at low-density having higher levels of molecular clock dysregulation. Conclusions Our results reveal the wide-scale impact of stocking density on transcriptional responses to infection and highlight the need to incorporate circadian biology into our understanding of disease dynamics in managed animals.
Fichier principal
Vignette du fichier
s12864-018-5098-7.pdf (962.41 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY - Paternité

Dates et versions

hal-04080731 , version 1 (07-06-2023)

Licence

Paternité

Identifiants

Citer

Amy Ellison, Tamsyn Uren Webster, Olivier REY, Carlos Garcia de Leaniz, Sofia Consuegra, et al.. Transcriptomic response to parasite infection in Nile tilapia (Oreochromis niloticus) depends on rearing density. BMC Genomics, 2018, 19 (1), pp.723. ⟨10.1186/s12864-018-5098-7⟩. ⟨hal-04080731⟩
7 Consultations
1 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More