Physcomitrium patens SMXL homologs are PpMAX2-dependent negative regulators of growth - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2023

Physcomitrium patens SMXL homologs are PpMAX2-dependent negative regulators of growth

Résumé

SMXL proteins are a plant-specific clade of type I HSP100/Clp-ATPases. SMXL genes are found in virtually all land plant genomes. However, they have mainly been studied in angiosperms. In Arabidopsis thaliana, three SMXL functional subclades have been identified: SMAX1/SMXL2, SMXL345 and SMXL678. Out of these, two subclades ensure transduction on endogenous hormone signals: SMAX1/SMXL2 are involved in KAI2-ligand (KL) signaling, while SMXL678 are involved in strigolactones (SLs) signaling. Many questions remain regarding the mode of action of these proteins, as well as their ancestral role. In light of recent discoveries in the liverwort Marchantia polymorpha, we addressed this second question by investigating the function of the four SMXL genes of the moss Physcomitrium patens. We demonstrate that PpSMXL proteins are negative regulators of growth, involved in the likely conserved ancestral MAX2-dependent KL signaling pathway. However, PpSMXL proteins expressed in A. thaliana unexpectedly cannot replace SMAX1/SMXL2 function in KL signaling, whereas they can functionally replace SMXL4/5 and restore root growth. Therefore, the molecular function of SMXL could be conserved, but not their interaction network. Moreover, one PpSMXL clade also positively regulates transduction of the SL signal in P. patens, this function most probably having an independent evolutionary origin to angiosperms SMXL678.
Fichier principal
Vignette du fichier
2023.04.11.536386v1.full(1).pdf (4.08 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04070429 , version 1 (15-04-2023)

Identifiants

Citer

Ambre Guillory, Mauricio Lopez-Obando, Khalissa Bouchenine, Philippe Le Bris, Alain Lecureuil, et al.. Physcomitrium patens SMXL homologs are PpMAX2-dependent negative regulators of growth. 2023. ⟨hal-04070429⟩
46 Consultations
8 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More