Specific targeting of inflammatory osteoclastogenesis by the probiotic yeast S. boulardii CNCM I-745 reduces bone loss in osteoporosis
Maria Bernadette Madel
(1)
,
Julia Halper
(1)
,
Lidia Ibáñez
,
Lozano Claire
(2)
,
Matthieu Rouleau
(1)
,
Antoine Boutin
(1)
,
Adrien Mahler
(1)
,
Rodolphe Pontier-Bres
(3)
,
Thomas Ciucci
,
Majlinda Topi
(3)
,
Christophe Hue
(4)
,
Jérôme Amiaud
,
Salvador Iborra
(5)
,
David Sancho
(6)
,
Dominique Heymann
,
Henri Jean Garchon
(4, 7)
,
Dorota Czerucka
(3)
,
Florence Apparailly
(8)
,
Isabelle Duroux-Richard
(2)
,
Abdelilah Wakkach
(3)
,
Claudine Blin-Wakkach
(3)
1
UniCA -
Université Côte d'Azur
2 Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB)
3 LIA ROPSE - Laboratoire International Associé Réponse des Organismes et Populations face au Stress Environnemental - Université Côte d’Azur - Centre Scientifique de Monaco
4 2I - Infection et inflammation
5 UCM - Universidad Complutense de Madrid = Complutense University of Madrid [Madrid]
6 CNIC - Centro Nacional de Investigaciones Cardiovasculares Carlos III [Madrid, Spain]
7 Hôpital Ambroise Paré [AP-HP]
8 Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB)
2 Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB)
3 LIA ROPSE - Laboratoire International Associé Réponse des Organismes et Populations face au Stress Environnemental - Université Côte d’Azur - Centre Scientifique de Monaco
4 2I - Infection et inflammation
5 UCM - Universidad Complutense de Madrid = Complutense University of Madrid [Madrid]
6 CNIC - Centro Nacional de Investigaciones Cardiovasculares Carlos III [Madrid, Spain]
7 Hôpital Ambroise Paré [AP-HP]
8 Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB)
Julia Halper
- Fonction : Auteur
- PersonId : 1247241
- ORCID : 0000-0003-3967-6633
Lidia Ibáñez
- Fonction : Auteur
- PersonId : 1247242
- ORCID : 0000-0002-2217-8817
Thomas Ciucci
- Fonction : Auteur
Jérôme Amiaud
- Fonction : Auteur
Salvador Iborra
- Fonction : Auteur
- PersonId : 1247243
- ORCID : 0000-0002-1607-1749
David Sancho
- Fonction : Auteur
- PersonId : 1247244
- ORCID : 0000-0003-2890-3984
Dominique Heymann
- Fonction : Auteur
Henri Jean Garchon
- Fonction : Auteur
- PersonId : 1199287
Florence Apparailly
- Fonction : Auteur
- PersonId : 749707
- IdHAL : florence-apparailly
- ORCID : 0000-0003-1762-084X
- IdRef : 117280674
Isabelle Duroux-Richard
- Fonction : Auteur
- PersonId : 21354
- IdHAL : isabelle-duroux-richard
Résumé
Bone destruction is a hallmark of chronic inflammation, and bone-resorbing osteoclasts arising under such a condition differ from steady-state ones. However, osteoclast diversity remains poorly explored. Here, we combined transcriptomic profiling, differentiation assays and in vivo analysis in mouse to decipher specific traits for inflammatory and steady-state osteoclasts. We identified and validated the pattern-recognition receptors (PRR) Tlr2, Dectin-1, and Mincle, all involved in yeast recognition as major regulators of inflammatory osteoclasts. We showed that administration of the yeast probiotic Saccharomyces boulardii CNCM I-745 (Sb) in vivo reduced bone loss in ovariectomized but not sham mice by reducing inflammatory osteoclastogenesis. This beneficial impact of Sb is mediated by the regulation of the inflammatory environment required for the generation of inflammatory osteoclasts. We also showed that Sb derivatives as well as agonists of Tlr2, Dectin-1, and Mincle specifically inhibited directly the differentiation of inflammatory but not steady-state osteoclasts in vitro. These findings demonstrate a preferential use of the PRR-associated costimulatory differentiation pathway by inflammatory osteoclasts, thus enabling their specific inhibition, which opens new therapeutic perspectives for inflammatory bone loss.
Domaines
Sciences du Vivant [q-bio]Format du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Specific targeting of inflammatory osteoclastogenesis by the probiotic yeast S. boulardii CNCM I-745 reduces bone loss in osteoporosis
|
Résumé |
en
Bone destruction is a hallmark of chronic inflammation, and bone-resorbing osteoclasts arising under such a condition differ from steady-state ones. However, osteoclast diversity remains poorly explored. Here, we combined transcriptomic profiling, differentiation assays and in vivo analysis in mouse to decipher specific traits for inflammatory and steady-state osteoclasts. We identified and validated the pattern-recognition receptors (PRR) Tlr2, Dectin-1, and Mincle, all involved in yeast recognition as major regulators of inflammatory osteoclasts. We showed that administration of the yeast probiotic Saccharomyces boulardii CNCM I-745 (Sb) in vivo reduced bone loss in ovariectomized but not sham mice by reducing inflammatory osteoclastogenesis. This beneficial impact of Sb is mediated by the regulation of the inflammatory environment required for the generation of inflammatory osteoclasts. We also showed that Sb derivatives as well as agonists of Tlr2, Dectin-1, and Mincle specifically inhibited directly the differentiation of inflammatory but not steady-state osteoclasts in vitro. These findings demonstrate a preferential use of the PRR-associated costimulatory differentiation pathway by inflammatory osteoclasts, thus enabling their specific inhibition, which opens new therapeutic perspectives for inflammatory bone loss.
|
Auteur(s) |
Maria Bernadette Madel
1
, Julia Halper
1
, Lidia Ibáñez
, Lozano Claire
2
, Matthieu Rouleau
1
, Antoine Boutin
1
, Adrien Mahler
1
, Rodolphe Pontier-Bres
3
, Thomas Ciucci
, Majlinda Topi
3
, Christophe Hue
4
, Jérôme Amiaud
, Salvador Iborra
5
, David Sancho
6
, Dominique Heymann
, Henri Jean Garchon
4, 7
, Dorota Czerucka
3
, Florence Apparailly
8
, Isabelle Duroux-Richard
2
, Abdelilah Wakkach
3
, Claudine Blin-Wakkach
3
1
UniCA -
Université Côte d'Azur
( 1039632 )
- Parc Valrose, 28, avenue Valrose 06108 Nice Cedex 2
- France
2
Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB)
( 1100747 )
- Institute for Regenerative Medicine and Biotherapy - 80 rue Augustin Fliche 34295 Montpellier - Cedex 5
- France
3
LIA ROPSE -
Laboratoire International Associé Réponse des Organismes et Populations face au Stress Environnemental - Université Côte d’Azur - Centre Scientifique de Monaco
( 1079033 )
- France
4
2I -
Infection et inflammation
( 470602 )
- Batiment Simone Veil - 2 avenue de la source de la Bièvre 78180 Montigny-le-Bretonneux
- France
5
UCM -
Universidad Complutense de Madrid = Complutense University of Madrid [Madrid]
( 48181 )
- Avda. de Séneca, 2, Ciudad Universitaria, 28040 Madrid
- Espagne
6
CNIC -
Centro Nacional de Investigaciones Cardiovasculares Carlos III [Madrid, Spain]
( 558780 )
- Calle de Melchor Fernández Almagro, 3, 28029 Madrid, Espagne
- Espagne
7
Hôpital Ambroise Paré [AP-HP]
( 300151 )
- 9, avenue Charles de Gaulle92104 Boulogne-Billancourt cedex
- France
8
Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB)
( 443668 )
- Institute for Regenerative Medicine and Biotherapy - 80 rue Augustin Fliche 34295 Montpellier - Cedex 5
- France
|
Audience |
Internationale
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Langue du document |
Anglais
|
Nom de la revue |
|
Date de publication |
2023
|
Volume |
12
|
Licence |
CC0 - Transfert dans le Domaine Public
|
Financement |
|
Domaine(s) |
|
Projet(s) ANR |
|
DOI | 10.7554/eLife.82037 |
Pubmed Id | 36848406 |
Origine :
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