PURPOSE: Evidence is emerging that tibio-femoral compartmental balancing is related to clinical outcomes after total knee arthroplasty (TKA). The purpose of this study was to assess if kinematic alignment (KA) delivered a balanced knee in flexion and extension after TKA on varus deformity, compared to functional alignment (FA). METHODS: This single-centre retrospective cohort study assessed 110 consecutive TKAs performed with an image-based robotic system for pre-operative varus deformity. The ligament balancing in the medial and lateral femorotibial compartments was assessed intra-operatively with a robotic system to evaluate if a KA plan would deliver a balanced knee. Balance was considered achieved if tibio-femoral compartments (medial/lateral) were equal to or less than 1.5mm, or if the estimated final gap position more than 2mm from the global implant thickness (17mm). Implant positioning was modified within limits previously defined for a FA philosophy to achieve balancing. Resection thickness and implant positioning were compared with the KA plan and after the FA adjustments; and also, between the patients with a final balanced knee in extension and at 90° of flexion.RESULTS: A total of 102 patients were eligible for final analysis. Mean age was 67.3?±?8.2years, average BMI was 29.1?±?5.2kg/m2, mean pre-operative coronal alignment was 174.0°?±?3.3° and sagittal alignment 3.4°?±?5.9°. Mean post-operative coronal alignment was 177.7°?±?1.0° and sagittal alignment 0.8°?±?1.9°. A KA plan delivered medial-lateral tibio-femoral compartment balancing in 67 cases (65.7%) for the extension gap and in 50 cases (49.1%) for the flexion gap. All measured bone resection depths were significantly less for FA compared to KA. To achieve balancing targets, final femoral component position was more externally rotated relative to the posterior condylar axis, (0.5° with KA compared to 1.7° with FA (p?