This study investigates the feasibility of using Acetone-Butanol-Ethanol mixture (ABE) as a function of the volumetric ratios (20%v, 30%v, 40%v, 50%v blended with n-dodecane) in order to determine the most suitable ABE ratio for CI engines in comparison to a butanol/n-dodecane blend. The “New One Shot Engine” set-up was used to determine the lift-off length (LOL), ignition delay (ID) and soot concentration by means of OH* chemiluminescence and Diffused Back Illumination imaging at an ambient pressure and temperature of 60 bar and 900 K corresponding to ECN Spray-A conditions. It was found that ID and LOL increased, while soot concentration was reduced with the increase in concentration of ABE and Butanol in dodecane blends. The blends with the smallest content (20%) of ABE or Bu exhibited similar combustion characteristics to n-dodecane with a considerable reduction in soot, indicating that these blends can be considered as a Diesel substitute without any engine modifications.