Filopodia-like protrusions of adjacent somatic cells shape the developmental potential of oocytes - Archive ouverte HAL Access content directly
Journal Articles Life Science Alliance Year : 2023

Filopodia-like protrusions of adjacent somatic cells shape the developmental potential of oocytes

Morgane Belle
Alain Chédotal

Abstract

The oocyte must grow and mature before fertilization, thanks to a close dialogue with the somatic cells that surround it. Part of this communication is through filopodia-like protrusions, called transzonal projections (TZPs), sent by the somatic cells to the oocyte membrane. To investigate the contribution of TZPs to oocyte quality, we impaired their structure by generating a full knockout mouse of the TZP structural component myosin-X (MYO10). Using spinning disk and super-resolution microscopy combined with a machine-learning approach to phenotype oocyte morphology, we show that the lack of Myo10 decreases TZP density during oocyte growth. Reduction in TZPs does not prevent oocyte growth but impairs oocyte-matrix integrity. Importantly, we reveal by transcriptomic analysis that gene expression is altered in TZP-deprived oocytes and that oocyte maturation and subsequent early embryonic development are partially affected, effectively reducing mouse fertility. We propose that TZPs play a role in the structural integrity of the germline–somatic complex, which is essential for regulating gene expression in the oocyte and thus its developmental potential.
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Dates and versions

hal-04043467 , version 1 (23-03-2023)

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Flora Crozet, Gaëlle Letort, Rose Bulteau, Christelle da Silva, Adrien Eichmuller, et al.. Filopodia-like protrusions of adjacent somatic cells shape the developmental potential of oocytes. Life Science Alliance, 2023, 6 (6), pp.e202301963. ⟨10.26508/lsa.202301963⟩. ⟨hal-04043467⟩
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