Localization of a human nucleoporin 155 gene (NUP155) to the 5p13 region and cloning of its cDNA - Archive ouverte HAL Access content directly
Journal Articles Genomics Year : 1999

Localization of a human nucleoporin 155 gene (NUP155) to the 5p13 region and cloning of its cDNA

Xiuqing Zhang
  • Function : Author
Huanming Yang
  • Function : Author
Morten J. Corydon
  • Function : Author
Xiaoxiao Zhang
  • Function : Author
Soren Pedersen
  • Function : Author
Julie R. Korenberg
  • Function : Author
Xiao-Ning Chen
  • Function : Author
Niels Gregersen
  • Function : Author
Erik Niebuhr
  • Function : Author
Guoyang Liu
  • Function : Author
Lars Bolund
  • Function : Author

Abstract

Nucleoporins are the main components of nuclear pore complexes (NPCs) involved in nucleo-cytoplasmic transport. Starting with an expressed DNA fragment retrieved by exon trapping from pooled human BAC clones mapped to the short arm of chromosome 5, we identified a human nucleoporin cDNA sequence by PCR from a human testis cDNA library. The coding sequence showed high homology to that of the rat nucleoporin 155 (Nup155) cDNA. FISH analysis with the human BAC clone as probe localized the human NUP155 gene to chromosome band 5p13. Northern analysis showed that the human NUP155 gene was expressed at different levels in all tissues tested. Two species of transcripts were observed with estimated lengths of 5.4 and 4.7 kb, respectively, in concordance with the finding of two alternative polyadenylation sites in the cDNA. The genomic location of the human NUP155 gene suggests a possible role in the mental and developmental retardation associated with hemizygous deletions of the 5p13 region.

Dates and versions

hal-04033187 , version 1 (16-03-2023)

Identifiers

Cite

Xiuqing Zhang, Huanming Yang, Morten J. Corydon, Xiaoxiao Zhang, Soren Pedersen, et al.. Localization of a human nucleoporin 155 gene (NUP155) to the 5p13 region and cloning of its cDNA. Genomics, 1999, 57 (1), pp.144-151. ⟨10.1006/geno.1999.5741⟩. ⟨hal-04033187⟩
5 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More