The aim of this study is to provide details on diet and hygienic conditions of the citizens of Ainos (modern Enez) during the Ottoman Empire from the 16th to the 19th centuries. For this we analysed a septic tank discovered during archaeological excavations in 2021. We conducted archaeobotanical and palynological studies on the deposits of the latrine and compared them to historical sources. The material obtained is very rich in fruits and seeds. Remains of grapes, figs, cherries and plums dominate the assemblage. Frequent fish remains indicate fish consumption. Wide range of pollen of insect-pollinated plants are proof of honey in the diet. The presence of fly pupae of Thoracochaeta zosterae strongly suggest that the construction was filled with a liquid, nutrient-rich content. Intestinal parasites document that humans were infected by a range of parasites, such as roundworm, whipworm and lancet liver fluke, providing further insights on poor hygienic conditions.