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Journal Articles Nucleic Acids Research Year : 1999

Gene targeting restricted to mouse striated muscle lineage

Abstract

Spatially and temporally regulated somatic mutations can be achieved by using the Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts restricted to striated muscle, we generated a transgenic mouse line expressing Cre recombinase under the control of the human α-skeletal actin promoter. Specific excision of a loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a pattern very similar to the expression of the endogenous α-skeletal actin gene. Therefore, the reported transgenic line can be used to target inactivation or activation of a given gene to the skeletal muscle lineage.

Dates and versions

hal-04026890 , version 1 (13-03-2023)

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Pierre Miniou, Danilo Tiziano, Tony Frugier, Natacha Roblot, Marianne Le Meur, et al.. Gene targeting restricted to mouse striated muscle lineage. Nucleic Acids Research, 1999, 27 (19), pp.27-30. ⟨10.1093/nar/27.19.e27⟩. ⟨hal-04026890⟩
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