Chapitre D'ouvrage Année : 2007

Estimating Thymic Function Through Quantification of T-Cell Receptor Excision Circles

Résumé

Analysis of immune reconstitution is of major importance in clinical settings such as following bone marrow transplantation or during anti-retroviral treatment of HIV-infected patients. In these patients, thymic function is essential for the reconstitution of a diversified T-cell receptor (TCR) repertoire. During thymopoiesis, several genetic rearrangements lead to the generation of fully functional TCR. By-products of these processes, the T-cell receptor excision circles (TRECs), are present in cells exported from the thymus but do not replicate during mitosis; they can thus be used as molecular markers for recent thymic emigrants. We demonstrate how thymic function can be assessed in a quantitative and noninvasive fashion in humans by estimating intrathymic precursor T-cell proliferation through the quantification of distinct TREC molecules in peripheral blood cells.

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Dates et versions

hal-04008357 , version 1 (28-02-2023)

Identifiants

Citer

Marie-Lise Dion, Rafick-Pierre Sékaly, Rémi Cheynier. Estimating Thymic Function Through Quantification of T-Cell Receptor Excision Circles. Immunological Tolerance, 380, Springer Nature, pp.197-213, 2007, Methods in Molecular Biology™, 978-1-58829-652-8. ⟨10.1007/978-1-59745-395-0_12⟩. ⟨hal-04008357⟩

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