Development of a set of SNP markers for population genetics of the sea peach (Halocynthia papillosa)
Résumé
A set of single nucleotide polymorphism (SNP) markers was developed from the transcriptome of the solitary ascidian Halocynthia papillosa. 1399 putative SNPs with coverage larger than 100 reads were identified, and a selected set of 320 SNPs was tested using a MassARRAY System on 95 samples from the NW Mediterranean. A total of 175 SNPs were successfully genotyped, polymorphic and in Hardy-Weinberg equilibrium over all samples. The newly developed loci will be a valuable tool for population genetics studies of this endemic ascidian species of the Mediterranean Sea.
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