Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic β- and α-Cells - Archive ouverte HAL Access content directly
Journal Articles Journal of Biological Chemistry Year : 2015

Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic β- and α-Cells

Ramkumar Mohan
  • Function : Author
Yiping Mao
  • Function : Author
Shungang Zhang
  • Function : Author
Yu-Wei Zhang
  • Function : Author
Cheng-Ran Xu
  • Function : Author
Xiaoqing Tang
  • Function : Author
  • PersonId : 1231279

Abstract

Insulin secreted from pancreatic β-cells and glucagon secreted from pancreatic α-cells are the two major hormones working in the pancreas in an opposing manner to regulate and maintain a normal glucose homeostasis. How microRNAs (miRNAs), a population of non-coding RNAs so far demonstrated to be differentially expressed in various types of cells, regulate gene expression in pancreatic β-cells and its closely associated α-cells is not completely clear. In this study, miRNA profiling was performed and compared between pancreatic β-cells and their partner α-cells. One novel miRNA, miR-483, was identified for its highly differential expression in pancreatic β-cells when compared to its expression in α-cells. Overexpression of miR-483 in β-cells increased insulin transcription and secretion by targeting SOCS3, a member of suppressor of cytokine signaling family. In contrast, overexpression of miR-483 decreased glucagon transcription and secretion in α-cells. Moreover, overexpressed miR-483 protected against proinflammatory cytokine-induced apoptosis in β-cells. This correlates with a higher expression level of miR-483 and the expanded β-cell mass observed in the islets of prediabetic db/db mice. Together, our data suggest that miR-483 has opposite effects in α- and β-cells by targeting SOCS3, and the imbalance of miR-483 and its targets may play a crucial role in diabetes pathogenesis.
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Dates and versions

hal-04004363 , version 1 (24-02-2023)

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Ramkumar Mohan, Yiping Mao, Shungang Zhang, Yu-Wei Zhang, Cheng-Ran Xu, et al.. Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic β- and α-Cells. Journal of Biological Chemistry, 2015, 290 (32), pp.19955-19966. ⟨10.1074/jbc.M115.650705⟩. ⟨hal-04004363⟩
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