Culturing Diatoms
La Culture des diatomées
Résumé
In this chapter, we provide information on cultures of diatoms, starting with a brief introduction of the history of diatom culture collections. We present a synopsis of diatom strains currently available in culture collections and their representation in terms of habitat diversity, geographical distribution, phylogeny and taxonomic diversity. We outline the major techniques for isolating and cultivating diatoms and discuss the problems and possible strategies linked to diatom life cycles and habitat requirements. We provide an overview of available techniques for genetic transformation of diatoms and strategies for forward genetics. Finally, we summarize information and techniques available for long term preservation of diatom cultures, focusing on cryopreservation.
Domaines
Sciences de l'environnement
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