An ultrasensitive hairpin sensor based on g-C3N4 nanocomposite for the detection of miRNA-155 in breast cancer patient serum - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Analytical and Bioanalytical Chemistry Année : 2022

An ultrasensitive hairpin sensor based on g-C3N4 nanocomposite for the detection of miRNA-155 in breast cancer patient serum

Résumé

Achieving the early diagnosis of breast cancer, through ultrasensitive detection of tumor marker miRNA-155, is a significant challenge. Therefore, an ultrasensitive hairpin electrochemical biosensor based on graphite-like phase carbon nitride composite was proposed. In this paper, poly(D-glucosamine) (PDG) was used as a stabilizer and reducing agent to prepare gold nanoparticles at room temperature, and then a graphite-like phase with a two-dimensional lamellar structure carbon nitride was further combined with it to obtain the poly(D-glucosamine)/gold nanoparticles/graphite-like phase carbon nitride nanocomposite (PDG/AuNPs/g-C3N4), in order to achieve the goal of signal amplification. The specific hairpin capture probe (HP) that recognized and bound miRNA-155 was then grafted. The hairpin biosensor showed a linear range of 0.1 fM–1 pM with a detection limit of 0.05 fM using differential pulse voltammetry (DPV) electrochemical analysis. Furthermore, the excellent performance hairpin electrochemical biosensor had been applied to the detection of miRNA-155 in human serum samples with good recovery.
Fichier non déposé

Dates et versions

hal-03997276 , version 1 (20-02-2023)

Identifiants

Citer

Pingping Peng, Sheng Wang, Siyuan Cai, Jing Cheng, Dan Tao, et al.. An ultrasensitive hairpin sensor based on g-C3N4 nanocomposite for the detection of miRNA-155 in breast cancer patient serum. Analytical and Bioanalytical Chemistry, 2022, 414 (24), pp.7325-7334. ⟨10.1007/s00216-022-04284-0⟩. ⟨hal-03997276⟩
15 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More