A novel electrochemical immunosensor for ultrasensitive detection of tumor necrosis factor α based on polystyrene -PAMAM dendritic polymer blend nanofibers
Résumé
A simple, rapid, and highly sensitive label-free immunosensor based on polystyrene (PS)-polyamidoamine (PAMAM) dendritic polymer nanofibers (NFs) for detection of tumor necrosis factor-α (TNF-α) cytokine was developed. The choice of PAMAM was due to the possibility of designing the surface of NFs to create stabilization of the antibody on the nanofiber surface. The immunosensor had a very high affinity for TNFα cytokines, with a linear response ranging from 10 to 200 pg/mL and a low limit of detection (LOD) of 669 fg/mL. Interference results with Interleukin-10 (IL-10) cytokine showed that the immunosensor was extremely specific and may be utilized to detect cytokines in clinical diagnosis.
Origine | Fichiers produits par l'(les) auteur(s) |
---|