In situ isobaric lipid mapping by MALDI-ion mobility separation-mass spectrometry imaging
Résumé
Lipids are essential components which play critical structural, energy storage and signaling roles in living organisms. MALDI mass spectrometry imaging (MSI) has been widely employed for lipid mapping in biological and biomedical studies to reveal the 2-dimension or 3-dimension localization of important lipid species. However, due to the highly diverse lipid structures, one major challenge in MSI analysis is the separation of isomeric and isobaric lipid species sharing the same nominal m/z value. Here we applied the recently developed timsTOF fleX MALDI MSI platform which incorporates trapped ion mobility spectrometry (TIMS) to perform for the first time in situ mapping and identification of isobaric and isomeric lipids in the freshwater crustacean Gammarus fossarum.
Domaines
ChimieOrigine | Fichiers produits par l'(les) auteur(s) |
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