Spontaneous locomotion strongly influences the state of the hippocampal network and is critically important for spatial information coding. However, the intracellular determinants of CA1 pyramidal cells activation during locomotion are poorly understood. Here we recorded the membrane potential of CA1 pyramidal cells (PCs) while non-overtrained mice spontaneously alternated between periods of movement and immobility during a virtual spatial navigation task. We found opposite membrane polarization between bursting and regular firing CA1 PCs during movement. Regular firing CA1 PCs were more depolarized and fired at higher frequency during movement compared to immobility while bursting CA1 PCs, located deep in the CA1 pyramidal cell layer and preferentially inhibited during sharp wave ripples, were hyperpolarized during movement in a speed dependent manner. This speeddependent suppression of a subpopulation of CA1 PCs could enhance signal to noise ratio for efficient spatial coding during locomotion.