Assessing the quantification of acetylation in konjac glucomannan via ATR-FTIR and solid-state NMR spectroscopy - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Carbohydrate Polymers Année : 2022

Assessing the quantification of acetylation in konjac glucomannan via ATR-FTIR and solid-state NMR spectroscopy

Kash Bhullar
  • Fonction : Auteur
Michael I.M. Horgan
  • Fonction : Auteur
Ashley Le
  • Fonction : Auteur
David Fania
  • Fonction : Auteur
Richard Wuhrer
  • Fonction : Auteur
Valentina Razmovski-Naumovski
  • Fonction : Auteur
Kelvin Chan
  • Fonction : Auteur

Résumé

Dietary fiber like konjac glucomannan (KGM) is important in maintaining good human health. There is no established method for quantifying the average degree of acetylation DA of this polysaccharide. Polysaccharides are notoriously difficult to dissolve. In this study, KGM could not be fully dissolved in common solvents and was characterized in the solid state. ATR-FTIR spectroscopy enabled a fast qualitative assessment of acetylation, selective to the outer layer of KGM particles, and identifying excipients like magnesium stearate. Average DA was quantified for the first time with solid-state 13C NMR in KGM: semi-quantitative measurements on the same arbitrary scale by cross polarization (1 to 2 days) were calibrated with a few longer single-pulse excitation measurements (approximately 1 week). DA values ranged from 4 to 8% of the hexoses in the backbone, in agreement with previously reported values. This method could be used for quality control and standardization of KGM products.

Domaines

Chimie
Fichier principal
Vignette du fichier
2022 Carbohydr Polym accepted.pdf (5.11 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03990524 , version 1 (12-04-2023)

Identifiants

Citer

Kash Bhullar, Michael I.M. Horgan, Ashley Le, David Fania, Richard Wuhrer, et al.. Assessing the quantification of acetylation in konjac glucomannan via ATR-FTIR and solid-state NMR spectroscopy. Carbohydrate Polymers, 2022, 291, pp.119659. ⟨10.1016/j.carbpol.2022.119659⟩. ⟨hal-03990524⟩
41 Consultations
53 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More