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Communication Dans Un Congrès Année : 2022

Metabolomic responses of freshwater periphytic microbiome to combined stress of artificial light at night (ALAN) and benzalkonium chloride

Résumé

Urban activities can be a threat for ecosystem sustainability. For instance, there are numerous findings about the adverse effects of anthropogenic chemicals released from urban treatment plants on freshwater periphytic communities, which play key role in ecosystems function and associated services. Despite this increasing evidence, there a still a paucity of knowledge on the effect of chemicals combined to other anthropogenic stress. Among them, of particular concern is the Artificial Light At Night (ALAN). Thus, through ten days exposure of freshwater periphyton in controlled conditions, we have recently highlighted the combined effect of ALAN and benzalkonium chloride (i.e. main component of alcohol-based hand sanitizers) on photosynthetic function, diatoms morphology and pigment composition whereas the associated molecular/biochemical mechanisms/responses remained unknown. In this context, the present study aims to fill this gap of knowledge by providing metabolomic insights on this combined effect. To this end, following the same experiment the metabolome and the lipidome were characterized. In particular, high-resolution mass spectrometry based-untargeted metabolomics was implemented in order to provide a comprehensive picture of the microbial activities (i.e. molecular phenotype) and identify biochemical pathways involved in the physiological and morphological impairments. In addition, targeted analysis of key class of lipids (phospholipids, glycerolipids, fatty acids) was performed to highlight potential effect on energy storage and chloroplast/thylakoid membranes. Preliminary results show clear effect of both individual factors and their combination through the discovery of specific metabolome fingerprints and associated pathways that change over exposure time. Complementary analyses are still ongoing. The results highlight as well a shift in the composition of targeted lipid classes following exposure to (e.g. unsaturation rates of polar lipids). Overall, this study confirms the relevance of metabolomics/lipidomics approaches to provide mechanistic understanding of the response of environmental microbiomes to multiple stress, further supporting the discovery of biomarkers of ecosystem function impairment along the adverse outcomes pathway framework.
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Dates et versions

hal-03990007 , version 1 (15-02-2023)

Identifiants

  • HAL Id : hal-03990007 , version 1

Citer

Nicolas Creusot, Romain Vrba, Mélissa Eon, Nicolas Mazzella, Aurélie Moreira, et al.. Metabolomic responses of freshwater periphytic microbiome to combined stress of artificial light at night (ALAN) and benzalkonium chloride. EcotoxicoMic 2022 - Third International Conference on Microbial Ecotoxicology, Nov 2022, Montpellier, France. ⟨hal-03990007⟩
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