In this paper, we consider two-component mixture models having one single known component. This type of model is of particular interest when a known random phenomenon is contaminated by an unknown random effect. We propose in this setup to test the equality in distribution of the unknown random sources involved in two separate samples generated from such a model. For this purpose, we introduce the so-called IBM (Inversion-Best Matching) approach resulting in a tuning-free relaxed semiparametric Cramér-von Mises type two-sample test requiring minimal assumptions about the unknown distributions. The accomplishment of our work lies in the fact that we establish, under some natural and interpretable mutual-identifiability conditions specific to the two-sample case, a functional central limit theorem about the proportion parameters along with the unknown cumulative distribution functions of the model. An intensive numerical study is carried out from a large range of simulation setups to illustrate the asymptotic properties of our test. Finally, our testing procedure, implemented in the admix R package, is applied to a real-life situation through pairwise post COVID-19 mortality excess profile testing across a panel of European countries.