Stimulation of the immune system by a tumor antigen-bearing adenovirus-inspired VLP allows control of melanoma growth - Archive ouverte HAL Access content directly
Journal Articles Molecular Therapy - Methods and Clinical Development Year : 2023

Stimulation of the immune system by a tumor antigen-bearing adenovirus-inspired VLP allows control of melanoma growth

Abstract

Virus-like particles (VLPs) are versatile protein-based platforms that can be used as a vaccine platform mainly in infectiology. In the present work, we compared a previously designed, non-infectious, adenovirus-inspired 60-mer dodecahedric VLP to display short epitopes or a large tumor model antigen. To validate these two kinds of platforms as a potential immuno-stimulating approach, we evaluated their ability to control melanoma B16-ovalbumin (OVA) growth in mice. A set of adjuvants was screened, showing that polyinosinic-polycytidylic acid (poly(I:C)) was well suited to generate a homogeneous cellular and humoral response against the desired epitopes. In a prophylactic setting, vaccination with the VLP displaying these epitopes resulted in total inhibition of tumor growth 1 month after vaccination. A therapeutic vaccination strategy showed a delay in grafted tumor growth or its total rejection. If the "simple" epitope display on the VLP is sufficient to prevent tumor growth, then an improved engineered platform enabling display of a large antigen is a tool to overcome the barrier of immune allele restriction, broadening the immune response, and paving the way for its potential utilization in humans as an off-the-shelf vaccine.
Fichier principal
Vignette du fichier
Besson et al. Molecular therapy.pdf (2.05 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03969612 , version 1 (26-10-2023)

Identifiers

Cite

Solène Besson, Emilie Boucher, David Laurin, Olivier Manches, Caroline Aspord, et al.. Stimulation of the immune system by a tumor antigen-bearing adenovirus-inspired VLP allows control of melanoma growth. Molecular Therapy - Methods and Clinical Development, 2023, 28, pp.76-89. ⟨10.1016/j.omtm.2022.12.003⟩. ⟨hal-03969612⟩
1153 View
19 Download

Altmetric

Share

Gmail Facebook X LinkedIn More