During wastewater treatment, micropollutants are only partly eliminated and may present a risk for human health and aquatic ecosystems. The potential impacts these substances may have are currently underestimated due to the lack in available concentrations that lie below the limit of quantification (LOQ) for an important set of micropollutants. Here, the potential impacts due to 261 organic micropollutants on human health and aquatic environments were investigated at the scale of France. Even with concentrations below the LOQ, certain micropollutants were found to have a significant potential impact. For unmeasured concentrations, a global concentration distribution built from several datasets with different LOQ was used. By disregarding the unmeasured micropollutants, the potential impacts have been underestimated by more than 300% on both human health and aquatic environments. Certain substances, such as hydrazine, endrin, or 2,3,7,8-TetraCDD, could lead to very strong potential impacts, even with unmeasured concentration levels. Moreover, the usual convention of LOQ/2 to replace unmeasured concentrations also appeared to overestimate the potential impact. The present work can be adapted to any other compartment or geographical context.