Abstract Carbon dioxide is one of the leading contributors to global warming. Oxy-fuel combustion (OFC) integrated with carbon capture and storage (CCS) technology is an efficient way to reduce carbon dioxide emissions. In OFC, pure oxygen (O2) is used instead of air to react with hydrocarbon fuel. Consequently, the products of combustion mainly include carbon dioxide (CO2) and water vapor (H2O) under lean conditions. Meanwhile, due to the absence of N2 in the intake charge, nitrogen-related emissions such as NOx are greatly removed from the exhaust gases. In the present study, the effect of intake charge temperature on OFC has been investigated in a diesel engine under the homogeneous charge compression ignition (HCCI) mode. In order to control combustion temperature and avoid overheating problems caused by oxygen in OFC, a portion of the exhaust CO2 was added to the O2. For this purpose, different CO2 dilutions ranging from 79-85% have been employed. It has been found that OFC can significantly reduce CO and particulate matter (PM) emissions while eliminating NOx emissions. With a higher intake charge temperature, combustion occurs earlier with shorter main stages, reducing the indicated mean effective pressure (IMEP) and increasing the indicated specific fuel consumption (ISFC), whereas, with a lower intake charge temperature, combustion stability deteriorates leading to incomplete OFC. By raising the intake charge temperature from 140 °C to 220 °C and applying 21% O2 and 79% CO2 v/v, the indicated thermal efficiency (ITE) is reduced from 34.6% to 29.2% while ISFC is increased from 0.24 to 0.285 Kg/kWh.