The aim of this work was to study the elemental composition of the lyophilized latex obtained from Euphorbia milii var. hislopii (N.E.Br.) Ursch & Leandri) and the tegument of adult male of Schistosoma mansoni Sambon, 1907 originated from cercariae previously exposed to the latex. The composition and concentrations of 15 elements were characterized using scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The Magnesium, Potassium and Rhodium were found in high weight percent in the lyophilized latex. High levels of Sodium, Nitrogen and Magnesium elements were detected respectively in control adult male S. mansoni. The cercariae exposition of S. mansoni to the E. milii latex changed the elemental composition of adult parasite, which showed higher level of Nitrogen instead of Sodium as observed in normal parasites. Furthermore the comparison of elements of the control group and the adult worms from cercariae exposed to the latex showed a significant decrease in Nitrogen and increase in Sodium, Potassium and Magnesium. The present work represents the first analysis of the elemental composition of the lyophilized latex of E. milii var. hislopii and its impact in the male S. mansoni by Electron Probe X-Ray Microanalysis