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              <p>Understanding microplastic particles (MPs) accumulation and transport along rivers represents a major task due to the complexity and heterogeneity of rivers, and their interactions with their wider corridor. The identification of MPs hotspots and their potential sources is especially challenging in coarse-bed rivers transporting a wide range of particle sizes with a high degree of variability in time and space. This research focuses on the gravel-bed Ain River (Rhoˆne River tributary, France) which is managed by means of various dams and also hosts one of the major plastic production centres in Europe (Oyonnax and Bienne Plastic Valleys). In this research, (i) Geographical Information Systems (GIS) were used to locate plastic factories and to characterise the land use of the Ain River watershed. (ii) On the field, sediment samples were extracted from the hyporheic zone (HZ) of mobile gravel bar heads, while hydro-sedimentary settings were measured in order to describe site conditions. Sampling sites were especially established in downwelling areas (i.e. where the surface water entered the hyporheic zone), upstream and downstream of dams and plastic factories. (iii) After density separation and organic matter digestion of sediment, MPs were characterised with a μFTIR device followed by data processing via the siMPle software. This work highlighted the trapping efficiency of alluvial bars for MPs. The highest MPs concentrations were found along the Plastic Valleys (up to 4400 MPs/kg), while the lower river was less contaminated by MPs. After grain-size correction, a significant breakpoint was identified in the area of the main dams, revealing their major influence on MPs distribution. The variability in MPs concentrations and types suggested a local origin for most of MPs. A particular feature was the dominance of polypropylene (PP) which appears as a critical industrial heritage as the studied region is specialised in the manufacturing of hard plastics. Indeed, multivariate analyses also revealed that MPs concentrations and types were mostly driven by the vicinity of plastic factories and urban areas. This relationship between the land use, the presence of dams and MPs characteristics provides key results for the MPs assessment and the improvement of management issues along coarse-bed rivers.</p>
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