FTIR and Autofluorescence spectroscopy to understand and predict properties of dilute acid pretreated poplar
Résumé
The transformation of lignocellulosic biomass (LB) to obtain chemicals, materials and biofuels through the establishment of biorefineries is in full development [1]. The difficulty in valuing this LB lies in its chemical and physical complexity, with cellulose, hemicelluloses and lignin forming a complex three-dimensional network [2]. In order to facilitate the accessibility, progression and action of the catalysts (notably enzymatic) in the lignocellulosic matrix, a physico-chemical pretreatment step of the LB is necessary [3]. Given the cost of pretreatment, it is critical to understand and predict their multiscale effects on LB.
In our project, we have designed a set of dilute acid pretreatment experiments on poplar wood aimed at covering a wide range of conditions related to time, temperature and acid concentration, generating no less than 32 different samples. Then each sample has been characterized for its chemical composition and spectral properties.
Spectral FTIR analysis shows the evolution of specific absorption bands related in particular to hemicelluloses which are degraded during pretreatment. Measurement of autofluorescence intensity indicates how the environment of lignin, which is known as a barrier to enzymatic activity, is altered. In both cases, correlations have been drawn between spectral markers and chemical properties to determine possible prediction tools of LB-pretreated chemical composition depending on pretreatment severity.
[1] Beig B, Riaz M, Naqvi SR, Hassan M, Zheng ZF, Karimi K, et al. Current challenges and innovative developments in pretreatment of lignocellulosic residues for biofuel production: A review. Fuel. 2021;287:18.
[2] Pu YQ, Hu F, Huang F, Davison BH, Ragauskas AJ. Assessing the molecular structure basis for biomass recalcitrance during dilute acid and hydrothermal pretreatments. Biotechnol Biofuels. 2013;6:13.
[3] Mankar AR, Pandey A, Modak A, Pant KK. Pretreatment of lignocellulosic biomass: A review on recent advances. Bioresour Technol. 2021;334:12.
Domaines
Sciences du Vivant [q-bio]
Origine : Fichiers produits par l'(les) auteur(s)
Licence : CC BY NC SA - Paternité - Pas d'utilisation commerciale - Partage selon les Conditions Initiales
Licence : CC BY NC SA - Paternité - Pas d'utilisation commerciale - Partage selon les Conditions Initiales