microsporidia are obligate intracellular pathogens capable of infecting a wide variety of hosts ranging from invertebrates to vertebrates. the infection process requires a step of prior adherence of microsporidia to the surface of host cells. a few studies demonstrated the involvement of proteins containing a ricin-b lectin (rbl) domain in parasite infection. in this study anncalia algerae and encephalitozoon cuniculi genomes were screened by bioinformatic analysis to identify proteins with an extracellular prediction and possessing rbl-type carbohydrate-binding domains, being both potentially relevant factors contributing to host cell adherence. three proteins named aarbll-1 and aarbll-2 from a. algerae and ecrbll-1 from e. cuniculi, were selected and comparative analysis of sequences suggested their belonging to a multigenic family, with a conserved structural rbl domain despite a significant amino acid sequence divergence. the production of recombinant proteins and antibodies against the three proteins allowed their subcellular localization on the spore wall and/or the polar tube. adherence inhibition assays based on pre-treatments with recombinant proteins or antibodies highlighted the significant decrease of the proliferation of both e. cuniculi and a. algerae, strongly suggesting that these proteins are involved in the infection process.