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Article Dans Une Revue Analytical and Bioanalytical Chemistry Année : 2023

Fabrication of a novel electrochemical biosensor based on a molecular imprinted polymer-aptamer hybrid receptor for lysozyme determination

Résumé

In this work, a novel, sensitive, and rapid electrochemical biosensor was employed to detect lysozyme (Lys) using a double receptor of molecular imprinted polymer (MIP)-aptamer. First, a glassy carbon electrode (GCE) was modified with a nanocomposite consisting of multi-wall carbon nanotubes (MWCNTs), nitrogen-doped carbon quantum dots (N-CQDs), and chitosan. Subsequently, aptamer (Apt)-Lys complex was immobilized on MWCNTs-N-CQDs-chitosan/GCE via binding between carboxyl groups present in the nanocomposite and the terminal amine groups of the aptamer. Following that, methylene blue monomer was electrochemically polymerized around the Apt-Lys complex on the MWCNTs-N-CQDs-chitosan/GCE surface. Finally, after the template removal, the remaining cavities along with the aptamers created a new hybrid receptor of MIP-aptamer. The MWCNTs-N-CQDs-chitosan nanocomposite could provide large amounts of carboxyl groups for binding to amino-functionalized aptamers, considerable electrical conductivity, and a high surface-to-volume ratio. These beneficial features facilitated the Apt-Lys complex immobilization and gave improved electrochemical signal. The obtained MIP-aptamer hybrid receptor allowed lysozyme determination even at concentrations as low as 4.26 fM within the functional range of 1 fM to 100 nM.
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Dates et versions

hal-03919028 , version 1 (02-01-2023)

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Tavoos Beiki, Ghasem Najafpour-Darzi, Maedeh Mohammadi, Mohsen Shakeri, Rabah Boukherroub. Fabrication of a novel electrochemical biosensor based on a molecular imprinted polymer-aptamer hybrid receptor for lysozyme determination. Analytical and Bioanalytical Chemistry, 2023, 415, pp.899-911. ⟨10.1007/s00216-022-04487-5⟩. ⟨hal-03919028⟩
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