Microbial sophorolipids inhibit colorectal tumour cell growth in vitro and restore haematocrit in Apc min+/- mice - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Applied Microbiology and Biotechnology Année : 2022

Microbial sophorolipids inhibit colorectal tumour cell growth in vitro and restore haematocrit in Apc min+/- mice

Breedge Callaghan
Matthew Twigg
Inge van Bogaert
Roger Marchant
Christopher Mitchell
Ibrahim Banat

Résumé

Sophorolipids are glycolipid biosurfactants consisting of a carbohydrate sophorose head with a fatty acid tail and exist in either an acidic or lactonic form. Sophorolipids are gaining interest as potential cancer chemotherapeutics due to their inhibitory effects on a range of tumour cell lines. Currently, most anti-cancer studies reporting the effects of sophorolipids have focused on lactonic preparations with the effects of acidic sophorolipids yet to be elucidated. We produced a 94% pure acidic sophorolipid preparation which proved to be non-toxic to normal human colonic and lung cells. In contrast we observed a dose-dependent reduction in viability of colorectal cancer lines treated with the same preparation. Acidic sophorolipids induced apoptosis and necrosis, reduced migration and inhibited colony formation in all cancer cell lines tested. Furthermore, oral administration of 50 mg kg-1 acidic sophorolipids over 70 days to Apc min+/-mice was well tolerated and resulted in an increased haematocrit, as well as reducing splenic size and red pulp area. Oral feeding did not affect tumour numbers or sizes in this model. This is the first study to show that acidic sophorolipids dose-dependently and specifically reduces colon cancer cell viability in addition to reducing tumour-associated bleeding in the Apc min+/-mouse model.

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Matériaux
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Dates et versions

hal-03911204 , version 1 (22-12-2022)

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Breedge Callaghan, Matthew Twigg, Niki Baccile, Inge van Bogaert, Roger Marchant, et al.. Microbial sophorolipids inhibit colorectal tumour cell growth in vitro and restore haematocrit in Apc min+/- mice. Applied Microbiology and Biotechnology, 2022, 106 (18), pp.6003-6016. ⟨10.1007/s00253-022-12115-6⟩. ⟨hal-03911204⟩
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