In Vitro Analysis of the Effects of ITER-Like Tungsten Nanoparticles: Cytotoxicity and Epigenotoxicity in BEAS-2B Cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nanomaterials Année : 2019

In Vitro Analysis of the Effects of ITER-Like Tungsten Nanoparticles: Cytotoxicity and Epigenotoxicity in BEAS-2B Cells

Chiara Uboldi
Marcos Sanles Sobrido
  • Fonction : Auteur
Elodie Bernard
  • Fonction : Auteur
Virginie Tassistro
  • Fonction : Auteur
Nathalie Herlin-Boime
  • Fonction : Auteur
Sébastien Garcia-Argote
  • Fonction : Auteur
Stéphane Roche
  • Fonction : Auteur
Fréderique Magdinier
Gheorghe Dinescu
Véronique Malard
Laurence Lebaron-Jacobs
  • Fonction : Auteur
Jerome Rose
  • Fonction : Auteur
Bernard Rousseau
  • Fonction : Auteur
Philippe Delaporte
  • Fonction : Auteur
Christian Grisolia
Thierry Orsière
  • Fonction : Auteur

Résumé

Tungsten was chosen as a wall component to interact with the plasma generated by the International Thermonuclear Experimental fusion Reactor (ITER). Nevertheless, during plasma operation tritiated tungsten nanoparticles (W-NPs) will be formed and potentially released into the environment following a Loss-Of-Vacuum-Accident, causing occupational or accidental exposure. We therefore investigated, in the bronchial human-derived BEAS-2B cell line, the cytotoxic and epigenotoxic effects of two types of ITER-like W-NPs (plasma sputtering or laser ablation), in their pristine, hydrogenated, and tritiated forms. Long exposures (24 h) induced significant cytotoxicity, especially for the hydrogenated ones. Plasma W-NPs impaired cytostasis more severely than the laser ones and both types and forms of W-NPs induced significant micronuclei formation, as shown by cytokinesis-block micronucleus assay. Single DNA strand breaks, potentially triggered by oxidative stress, occurred upon exposure to W-NPs and independently of their form, as observed by alkaline comet assay. After 24 h it was shown that more than 50% of W was dissolved via oxidative dissolution. Overall, our results indicate that W-NPs can affect the in vitro viability of BEAS-2B cells and induce epigenotoxic alterations. We could not observe significant differences between plasma and laser W-NPs so their toxicity might not be triggered by the synthesis method.

Dates et versions

hal-03911130 , version 1 (22-12-2022)

Identifiants

Citer

Chiara Uboldi, Marcos Sanles Sobrido, Elodie Bernard, Virginie Tassistro, Nathalie Herlin-Boime, et al.. In Vitro Analysis of the Effects of ITER-Like Tungsten Nanoparticles: Cytotoxicity and Epigenotoxicity in BEAS-2B Cells. Nanomaterials, 2019, 9 (9), pp.1233. ⟨10.3390/nano9091233⟩. ⟨hal-03911130⟩
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