Understanding the behaviour of cell metabolism is the crucial key in bioprocess development and optimization, as well as in the development of efficient therapies. Indeed, it is established that a cell’s behaviour relies on its genetics, and more practically on gene expression, and thus on metabolic pathways’ activity. There are currently two fundamental trends in the analysis of metabolic networks: the constraint-based modelling (CBM) approach for large-size networks, which is used to determine a space for a feasible metabolic steady-state flux solution, and the kinetic metabolic modelling approach for relatively small-size networks, which is used to study the dynamic behaviour of a regulated metabolic system.