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Pré-Publication, Document De Travail Année : 2022

The onset of molecule-spanning dynamics in a multi-domain protein

Benedikt Sohmen
  • Fonction : Auteur
Christian Beck
  • Fonction : Auteur
Tilo Seydel
Ingo Hoffmann
  • Fonction : Auteur
Bianca Hermann
  • Fonction : Auteur
Mark Nüesch
  • Fonction : Auteur
Marco Grimaldo
  • Fonction : Auteur
Frank Schreiber
  • Fonction : Auteur
Steffen Wolf
  • Fonction : Auteur
Felix Roosen-Runge
  • Fonction : Auteur
Thorsten Hugel
  • Fonction : Auteur

Résumé

Protein dynamics has been investigated on a wide range of time scales. Nano- and picosecond dynamics have been assigned to local fluctuations, while slower dynamics have been attributed to larger conformational changes. However, it is largely unknown how local fluctuations can lead to global allosteric changes. Here we show that molecule-spanning dynamics on the 100 ns time scale precede larger allosteric changes. We assign global real-space movements to dynamic modes on the 100 ns time scales, which became possible by a combination of single-molecule fluorescence, quasi-elastic neutron scattering and all-atom MD simulations. Additionally, we demonstrate the effect of Sba1, a co-chaperone of Hsp90, on these molecule-spanning dynamics, which implies functional importance of such dynamics. Our integrative approach provides comprehensive insights into molecule-spanning dynamics on the nanosecond time scale for a multi-domain protein and indicates that such dynamics are the molecular basis for allostery and large conformational changes in proteins.

Dates et versions

hal-03899376 , version 1 (14-12-2022)

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Benedikt Sohmen, Christian Beck, Tilo Seydel, Ingo Hoffmann, Bianca Hermann, et al.. The onset of molecule-spanning dynamics in a multi-domain protein. 2022. ⟨hal-03899376⟩
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