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Communication Dans Un Congrès Année : 2022

Development of biosensors for the assessment of seawater toxicity: Choice of the bioreporter

Eva Delaunay
  • Fonction : Auteur
  • PersonId : 1199647
Sulivan Jouanneau
Gerald Thouand

Résumé

The preservation of the oceans is a major issue of the 21st century. In 2000, the Water Framework Directive harmonized European regulations on water management to protect and restore the quality of aquatic environments, including the marine environment and the coast. If currently, few methods are available for the marine water management, there is a real need for global diagnostic tools for the evaluation of cellular toxicity. The aim of our study is to develop a microbial biosensor, using not just a single cell (like currently developed approaches) but a set of representative microorganisms from the autochthonous microbial population that receives the pollution. The principle of our approach is to determine the metabolic functions lost by the microbial community after an exposure to different families of toxics and to identify the bacterial families that carry these lost functions. The first step of this project aimed to expose the microbial community sampled from Roscoff (Finistère, France) to different pollutants characteristic of the marine ecosystem. For this, we have set up an exposure in microcosms. They were composed of sampled seawater exposed to different concentrations of anthracene, benzene, copper chloride, chlorpyrifos and PFOA. After 1 month of exposure, each condition was subject to Phenotype Microarrays for Microbial Cells technology from Biolog®. Thus, it was possible to determine the lost of metabolic functions regarding the cycle of carbon, nitrogen, sulfur and phosphorus following exposure. In parallel, the phenotypic profile of 40 bacterial strains from the same environment was determined with the same tool and for the same biogeochemical cycles. Specific phenotypic profiles were obtained for each of these strains. The selection of bioindicators was carried out thanks to the specific functions that they represent and which are lost following exposure in microcosms. The next step will be the integration of such microbial set into a bioassay and after a biosensor.
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Dates et versions

hal-03890583 , version 1 (08-12-2022)

Identifiants

  • HAL Id : hal-03890583 , version 1

Citer

Eva Delaunay, Sulivan Jouanneau, Marie-Jose Thouand Durand, Gerald Thouand. Development of biosensors for the assessment of seawater toxicity: Choice of the bioreporter. Ecotoxicomic2022, Nov 2022, Montpellier, France. ⟨hal-03890583⟩
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