Syngeneic Transplantation of Olfactory Ectomesenchymal Stem Cells Restores Learning and Memory Abilities in a Rat Model of Global Cerebral Ischemia - Archive ouverte HAL
N°Spécial De Revue/Special Issue Stem Cells International Année : 2018

Syngeneic Transplantation of Olfactory Ectomesenchymal Stem Cells Restores Learning and Memory Abilities in a Rat Model of Global Cerebral Ischemia

Antoine Veron
  • Fonction : Auteur
Cécile Bienboire-Frosini
  • Fonction : Auteur
Stéphane Girard
Kevin Sadelli
  • Fonction : Auteur
Michel Khrestchatisky
  • Fonction : Auteur
Jennifer Alexis
  • Fonction : Auteur
Patrick Pageat
  • Fonction : Auteur
Pietro Asproni
Manuel Mengoli
  • Fonction : Auteur
François Roman

Résumé

Stem cells are considered as promising tools to repair diverse tissue injuries. Among the different stem cell types, the “olfactory ectomesenchymal stem cells” (OE-MSCs) located in the adult olfactory mucosa stand as one of the best candidates. Here, we evaluated if OE-MSC grafts could decrease memory impairments due to ischemic injury. OE-MSCs were collected from syngeneic F344 rats. After a two-step global cerebral ischemia, inducing hippocampal lesions, learning abilities were evaluated using an olfactory associative discrimination task. Cells were grafted into the hippocampus 5 weeks after injury and animal’s learning abilities reassessed. Rats were then sacrificed and the brains collected for immunohistochemical analyses. We observed significant impairments in learning and memory abilities following ischemia. However, 4 weeks after OE-MSC grafts, animals displayed learning and memory performances similar to those of controls, while sham rats did not improve them. Immunohistochemical analyses revealed that grafts promoted neuroblast and glial cell proliferation, which could permit to restore cognitive functions. These results demonstrated, for the first time, that syngeneic transplantations of OE-MSCs in rats can restore cognitive abilities impaired after brain injuries and provide support for the development of clinical studies based on grafts of OE-MSCs in amnesic patients following brain injuries.
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hal-03888321 , version 1 (11-02-2024)

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Antoine Veron, Cécile Bienboire-Frosini, Stéphane Girard, Kevin Sadelli, Jean-Claude Stamegna, et al.. Syngeneic Transplantation of Olfactory Ectomesenchymal Stem Cells Restores Learning and Memory Abilities in a Rat Model of Global Cerebral Ischemia. Stem Cells International, 2018, pp.1-10, 2018, ⟨10.1155/2018/2683969⟩. ⟨hal-03888321⟩

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