Vehicle-to-Everything (5G-V2X) is a new emerging technology for vehicular networks standardized by 3GPP to contribute to road safety services. The flexibility of the New Radio (NR) frame structure is one of the most interesting 5G key features enabled by the use of the new 5G numerologies. The 5G numerologies are an enabler to meet the requirements of a wide range of 5G services. Indeed, V2X communication encompasses a variety of applications with different requirements. Therefore, the choice of the appropriate numerology for each V2X scenario is of particular importance. To this end, in this paper we present a comprehensive study of the impact of 5G numerologies on the performance of V2X applications. Using the Simu5G and 5GVLL simulators, we demonstrate that the choice of the appropriate numerology is a trade-off between the targeted requirements of V2X applications, the Inter-Carrier Interference (ICI) and the Inter-Symbol Interference (ISI) issues.