Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store depletion - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Cell Biology Année : 2004

Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store depletion

Susan Treves
  • Fonction : Auteur
Clara Franzini-Armstrong
  • Fonction : Auteur
Luca Moccagatta
  • Fonction : Auteur
Christophe Arnoult
  • Fonction : Auteur
Cristiano Grasso
  • Fonction : Auteur
Adam Schrum
  • Fonction : Auteur
Sylvie Ducreux
Michael Zhu
  • Fonction : Auteur
Katsuhiko Mikoshiba
  • Fonction : Auteur
Thierry Girard
  • Fonction : Auteur
Sophia Smida-Rezgui
  • Fonction : Auteur
Michel Ronjat
  • Fonction : Auteur
Francesco Zorzato
  • Fonction : Auteur

Résumé

In many cell types agonist-receptor activation leads to a rapid and transient release of Ca2+ from intracellular stores via activation of inositol 1,4,5 trisphosphate (InsP3) receptors (InsP3Rs). Stimulated cells activate store- or receptor-operated calcium channels localized in the plasma membrane, allowing entry of extracellular calcium into the cytoplasm, and thus replenishment of intracellular calcium stores. Calcium entry must be finely regulated in order to prevent an excessive intracellular calcium increase. Junctate, an integral calcium binding protein of endo(sarco)plasmic reticulum membrane, (a) induces and/or stabilizes peripheral couplings between the ER and the plasma membrane, and (b) forms a supramolecular complex with the InsP3R and the canonical transient receptor potential protein (TRPC) 3 calcium entry channel. The full-length protein modulates both agonist-induced and store depletion–induced calcium entry, whereas its NH2 terminus affects receptor-activated calcium entry. RNA interference to deplete cells of endogenous junctate, knocked down both agonist-activated calcium release from intracellular stores and calcium entry via TRPC3. These results demonstrate that junctate is a new protein involved in calcium homeostasis in eukaryotic cells.

Dates et versions

hal-03884456 , version 1 (05-12-2022)

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Citer

Susan Treves, Clara Franzini-Armstrong, Luca Moccagatta, Christophe Arnoult, Cristiano Grasso, et al.. Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store depletion. Journal of Cell Biology, 2004, 166 (4), pp.537-548. ⟨10.1083/jcb.200404079⟩. ⟨hal-03884456⟩
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