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Communication Dans Un Congrès Année : 2022

Combining phenotypic, metabolome and proteome data to study maize response to a mild nitrogen deficit

Isabelle Quilleré
Bertrand Hirel

Résumé

To decipher the biochemical bases of nitrogen (N) utilization and metabolism of silage maize in relation to growth and productivity, an untargeted metabolomic (proton NMR- and LC-QTOF-MS based) and proteomic approach was conducted on leaves of 29 hybrids cultivated in the field under optimal and reduced N fertilization. The corresponding biochemical data were analyzed either individually (PCA, ANOVA), or integrated with that of eco-physiological, developmental and yield- related traits (multi-block sparse PLS-DA). Such integrated analysis was conducted to interpret the underlying physiology concerning the plant response to a mild N deficit often occurring under agronomic conditions. The genetic diversity of the core panel of 29 European dent hybrids crossed to a flint tester was exploited to highlight common N-responsive metabolites and proteins in order to identify putative biological markers that could be used to pilot and rationalize N fertilization. The responses of metabolites, proteins, and yield-related traits to the reduced N treatment were also exploited to identify biochemical markers representative of a maize ideotype exhibiting better agronomic performances when N fertilization is limited. These markers could be used to select high- yielding commercial maize hybrids used for silage production requiring less N fertilizer inputs.
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Dates et versions

hal-03878405 , version 1 (29-11-2022)

Identifiants

  • HAL Id : hal-03878405 , version 1

Citer

Maria Urrutia, Melisande Blein-Nicolas, Olivier Fernandez, Stéphane Bernillon, Mickael Maucourt, et al.. Combining phenotypic, metabolome and proteome data to study maize response to a mild nitrogen deficit. Journées du Protéome Vert, Jun 2022, Gif-sur-Yette, France. ⟨hal-03878405⟩
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