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Pré-Publication, Document De Travail Année : 2021

Photoprotection is regulated by light-independent CO2 availability

M. Águila Ruiz-Sola
Serena Flori
Yizhong Yuan
Gaelle Villain
  • Fonction : Auteur
Emanuel Sanz-Luque
Petra Redekop
Ryutaro Tokutsu
Anika Kueken
Angeliki Tsichla
Georgios Kepesidis
  • Fonction : Auteur
Guillaume Allorent
  • Fonction : Auteur
Marius Arend
  • Fonction : Auteur
Fabrizio Iacono
Giovanni Finazzi
  • Fonction : Auteur
Michael Hippler
Zoran Nikoloski
Jun Minagawa
Arthur Grossman
Dimitris Petroutsos
  • Fonction : Auteur
  • PersonId : 1196143

Résumé

Abstract Photosynthetic algae cope with suboptimal levels of light and CO 2 . In low CO 2 and excess light, the green alga Chlamydomonas reinhardtii activates a CO 2 Concentrating Mechanism (CCM) and photoprotection; the latter is mediated by LHCSR1/3 and PSBS. How light and CO 2 signals converge to regulate photoprotective responses remains unclear. Here we show that excess light activates expression of photoprotection- and CCM-related genes and that depletion of CO 2 drives these responses, even in total darkness. High CO 2 levels, derived from respiration or impaired photosynthetic fixation, repress LHCSR3 and CCM genes while stabilizing the LHCSR1 protein. We also show that CIA5, which controls CCM genes, is a major regulator of photoprotection, elevating LHCSR3 and PSBS transcript accumulation while inhibiting LHCSR1 accumulation. Our work emphasizes the importance of CO 2 in regulating photoprotection and the CCM, demonstrating that the impact of light on photoprotection is often indirect and reflects intracellular CO 2 levels. Teaser Photoprotection- and CCM-related genes can be activated by changes in CO 2 availability even in the absence of light.

Dates et versions

hal-03876891 , version 1 (29-11-2022)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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M. Águila Ruiz-Sola, Serena Flori, Yizhong Yuan, Gaelle Villain, Emanuel Sanz-Luque, et al.. Photoprotection is regulated by light-independent CO2 availability. 2021. ⟨hal-03876891⟩
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