RAB6 and dynein drive post-Golgi apical transport to prevent neuronal progenitor delamination - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue EMBO Reports Année : 2022

RAB6 and dynein drive post-Golgi apical transport to prevent neuronal progenitor delamination

Jean‐baptiste Brault
Hugo Lachuer
Gaelle Boncompain
Richard Belvindrah
Fiona Francis
Franck Perez
Bruno Goud
Alexandre D Baffet

Résumé

Radial glial (RG) cells are the neural stem cells of the developing neocortex. Apical RG (aRG) cells can delaminate to generate basal RG (bRG) cells, a cell type associated with human brain expansion. Here, we report that aRG delamination is regulated by the post-Golgi secretory pathway. Using in situ subcellular live imaging, we show that post-Golgi transport of RAB6+ vesicles occurs toward the minus ends of microtubules and depends on dynein. We demonstrate that the apical determinant Crumbs3 (CRB3) is also transported by dynein. Double knockout of RAB6A/A' and RAB6B impairs apical localization of CRB3 and induces a retraction of aRG cell apical process, leading to delamination and ectopic division. These defects are phenocopied by knockout of the dynein activator LIS1. Overall, our results identify a RAB6-dynein-LIS1 complex for Golgi to apical surface transport in aRG cells, and highlights the role of this pathway in the maintenance of neuroepithelial integrity.
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Dates et versions

hal-03872734 , version 1 (25-11-2022)

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Jean‐baptiste Brault, Sabine Bardin, Marusa Lampic, Jacopo A Carpentieri, Laure Coquand, et al.. RAB6 and dynein drive post-Golgi apical transport to prevent neuronal progenitor delamination. EMBO Reports, 2022, 23, ⟨10.15252/embr.202254605⟩. ⟨hal-03872734⟩
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