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Article Dans Une Revue Cell Reports Methods Année : 2022

All-optical inter-layers functional connectivity investigation in the mouse retina

Résumé

We developed a multi-unit microscope for all-optical inter-layers circuits interrogation. The system performs two-photon (2P) functional imaging and 2P multiplexed holographic optogenetics at axially distinct planes. We demonstrated the capability of the system to map, in the mouse retina, the functional connectivity between rod bipolar cells (RBCs) and ganglion cells (GCs) by activating single or defined groups of RBCs while recording the evoked response in the GC layer with cell-type specificity and single-cell resolution. We then used a logistic model to probe the functional connectivity between cell types by deriving the “cellular receptive field” describing how RBCs impact each GC type. With the capability to simultaneously image and control neuronal activity at axially distinct planes, the system enables a precise interrogation of multi-layered circuits. Understanding this information transfer is a promising avenue to dissect complex neural circuits and understand the neural basis of computations.
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Dates et versions

hal-03871619 , version 1 (13-12-2022)

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Giulia Lia Beatrice Spampinato, Emiliano Ronzitti, Valeria Zampini, Ulisse Ferrari, Francesco Trapani, et al.. All-optical inter-layers functional connectivity investigation in the mouse retina. Cell Reports Methods, 2022, 2 (8), pp.100268. ⟨10.1016/j.crmeth.2022.100268⟩. ⟨hal-03871619⟩
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