A flexible two-photon fiberscope for fast activity imaging and precise optogenetic photostimulation of neurons in freely moving mice - Archive ouverte HAL Access content directly
Journal Articles Neuron Year : 2022

A flexible two-photon fiberscope for fast activity imaging and precise optogenetic photostimulation of neurons in freely moving mice

Nicolò Accanto
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François G.C. Blot
Valentina Emiliani

Abstract

We developed a flexible two-photon microendoscope (2P-FENDO) capable of all-optical brain interrogation at near cellular resolution in freely moving mice. The system performs fast two-photon (2P) functional imaging and 2P holographic photostimulation of single and multiple cells using axially confined extended spots. Proof-of-principle experiments were performed in freely moving mice co-expressing jGcaMP7s and the opsin ChRmine in the visual or barrel cortex. On a field of view of 250 µm in diameter, we demonstrated functional imaging at a frame rate of up to 50 Hz and precise photostimulation of selected groups of cells. With the capability to simultaneously image and control defined neuronal networks in freely moving animals, 2P-FENDO will enable to precisely define the functions of neurons in the brain and their interactions during naturalistic behaviours.
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Dates and versions

hal-03865947 , version 1 (30-11-2022)

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Nicolò Accanto, François G.C. Blot, Antonio Lorca-Cámara, Valeria Zampini, Florence Bui, et al.. A flexible two-photon fiberscope for fast activity imaging and precise optogenetic photostimulation of neurons in freely moving mice. Neuron, In press, ⟨10.1016/j.neuron.2022.10.030⟩. ⟨hal-03865947⟩
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