Mathematical Modeling of Intracellular Calcium in Presence of Receptor: A Homeostatic Model for Endothelial Cell
Résumé
Calcium is a ubiquitous molecule and second messenger that regulates many cellular functions ranging from exocytosis to cell proliferation at different time scales. In the vasculature, a constant adenosine triphosphate (ATP) concentration is maintained because of ATP released by red blood cells (RBCs). These ATP molecules continuously react with purinergic receptors on the surface of endothelial cells (ECs). Consequently, a cascade of chemical reactions are triggered that result in a transient cytoplasmic calcium (Ca 2+), followed by return to its basal concentration. The mathematical models proposed in literature are able to reproduce the transient peak. However, the trailing concentration is always higher than the basal cytoplasmic Ca 2+ concentrations, and the Ca 2+ concentration in endoplasmic reticulum (ER) remains lower than its initial concentration. This means that the intracellular homeostasis is not recovered. We propose, herein, a minimal model of calcium kinetics. We find that the desensitization of EC surface receptors due to phosphorylation and recycling plays a vital role in maintaining calcium homeostasis in the presence of a constant stimulus (ATP). The model is able to capture several experimental observations such as refilling of Ca 2+ in the ER, variation of cytoplasmic Ca 2+ transient peak in ECs, the resting cytoplasmic Ca 2+ concentration, the effect of removing ATP from the plasma on Ca 2+ homeostasis, and the saturation of cytoplasmic Ca 2+ transient peak with increase in ATP concentration. Direct confrontation with several experimental results is conducted.This work paves the way to systematic studies for coupling between blood flow and chemical signaling, and should contribute to a better understanding of the relation between (patho)physiological conditions and Ca 2+ kinetics.
Domaines
Biophysique [physics.bio-ph]Origine | Fichiers produits par l'(les) auteur(s) |
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