Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge Crambe tailliezi
Thi-Ngoc-Dung Nguyen
(1)
,
Omid Feizbakhsh
(1)
,
Estelle Sfecci
(2)
,
Blandine Baratte
(1, 3, 4)
,
Claire Delehouzé
(1)
,
Adrien Garcia
(2)
,
Corentin Moulin
(2)
,
Pierre Colas
(1)
,
Sandrine Ruchaud
(1)
,
Mohamed Mehiri
(2)
,
Stéphane Bach
(1, 3, 4)
Omid Feizbakhsh
- Fonction : Auteur
- PersonId : 1191483
Blandine Baratte
- Fonction : Auteur
- PersonId : 1191197
Claire Delehouzé
- Fonction : Auteur
- PersonId : 1109043
- ORCID : 0000-0003-4114-3051
- IdRef : 276240308
Pierre Colas
- Fonction : Auteur
- PersonId : 853040
Sandrine Ruchaud
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- Fonction : Auteur correspondant
- PersonId : 1060079
- IdHAL : sandrine-ruchaud
- ORCID : 0000-0002-4606-6625
- IdRef : 158557964
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Mohamed Mehiri
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- Fonction : Auteur correspondant
- PersonId : 1191486
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Stéphane Bach
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- Fonction : Auteur correspondant
- PersonId : 974150
- IdHAL : stephane-l-bach
- ORCID : 0000-0002-7186-7483
- IdRef : 131509802
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Résumé
Regulated cell death (RCD) results from the activation of one or more signal transduction modules both in physiological or pathological conditions. It is now established that RCD is involved in numerous human diseases, including cancer. As regulated cell death processes can be modulated by pharmacological tools, the research reported here aims to characterize new marine compounds acting as RCD modulators. Protein kinases (PKs) are key signaling actors in various RCDs notably through the control of either mitosis (e.g., the PKs Aurora A and B) or necroptosis (e.g., RIPK1 and RIPK3). From the primary screening of 27 various extracts of marine organisms collected in the Mediterranean Sea, an extract and subsequently a purified high molecular weight compound dubbed P3, were isolated from the marine sponge Crambe tailliezi and characterized as a selective inhibitor of PKs Aurora A and B. Furthermore, P3 was shown to induce apoptosis and to decrease proliferation and mitotic index of human osteosarcoma U-2 OS cells.
Format du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge Crambe tailliezi
|
Résumé |
en
Regulated cell death (RCD) results from the activation of one or more signal transduction modules both in physiological or pathological conditions. It is now established that RCD is involved in numerous human diseases, including cancer. As regulated cell death processes can be modulated by pharmacological tools, the research reported here aims to characterize new marine compounds acting as RCD modulators. Protein kinases (PKs) are key signaling actors in various RCDs notably through the control of either mitosis (e.g., the PKs Aurora A and B) or necroptosis (e.g., RIPK1 and RIPK3). From the primary screening of 27 various extracts of marine organisms collected in the Mediterranean Sea, an extract and subsequently a purified high molecular weight compound dubbed P3, were isolated from the marine sponge Crambe tailliezi and characterized as a selective inhibitor of PKs Aurora A and B. Furthermore, P3 was shown to induce apoptosis and to decrease proliferation and mitotic index of human osteosarcoma U-2 OS cells.
|
Auteur(s) |
Thi-Ngoc-Dung Nguyen
1
, Omid Feizbakhsh
1
, Estelle Sfecci
2
, Blandine Baratte
1, 3, 4
, Claire Delehouzé
1
, Adrien Garcia
2
, Corentin Moulin
2
, Pierre Colas
1
, Sandrine Ruchaud
1
, Mohamed Mehiri
2
, Stéphane Bach
1, 3, 4
1
LBI2M -
Laboratoire de Biologie Intégrative des Modèles Marins
( 542078 )
- Station Biologique de Roscoff Place Georges Teissier 29680 Roscoff
- France
2
ICN -
Institut de Chimie de Nice
( 212876 )
- Faculté des Sciences Parc Valrose 28 Avenue Valrose 06108 Nice cedex 2
- France
3
FR2424 -
Fédération de recherche de Roscoff
( 1147437 )
- Place Georges Teissier
29680 Roscoff
- France
4
SBR -
Station biologique de Roscoff = Roscoff Marine Station
( 541806 )
- Place Georges Teissier - BP 74 29682 ROSCOFF CEDEX
- France
|
Date de publication |
2019
|
Numéro |
10
|
Page/Identifiant |
569
|
Langue du document |
Anglais
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Volume |
17
|
Domaine(s) |
|
Projet(s) ANR |
|
Projet(s) Européen(s) |
|
Mots-clés |
en
kinase inhibition, regulated cell death, RIP1-independent cell death, marine natural products, Crambe tailliezi, Screening Analysis
|
DOI | 10.3390/md17100569 |
Origine :
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