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Article Dans Une Revue Biotechnology and Bioengineering Année : 2022

The architecture and metabolic traits of monospecific photosynthetic biofilms studied in a custom flow‐through system

Résumé

Microalgae biofilms have a great ecological importance and a high biotechnological potential. Nevertheless, an in-depth and combined structural (i.e. the architecture of the biofilm) and physiological characterization of microalgae biofilms is still missing. An approach able to provide at the same time physiological and structural information during biofilm growth would be of paramount importance to understand these complex biological systems and to optimize their productivity. In this study, monospecific biofilms of a diatom and a green alga were grown under dynamic conditions in custom flow cells represented by UV/Vis spectroscopic cuvettes. Such flow cells were conceived to characterize the biofilms by several techniques mostly in situ and in a non-destructive way. Physiological traits were obtained measuring variable chlorophyll a fluorescence by pulse amplitude modulated fluorometry (PAM) and by scanning the biofilms in a spectrometer to obtain in vivo pigments spectral signatures. The architectural features were obtained by imaging the biofilms with a confocal scanning laser microscope (CLSM) and an optical coherence tomograph (OCT). Overall, this experimental setup allowed us to follow the growth of two biofilm-forming microalgae showing that cell physiology is more affected in complex biofilms likely as a consequence of alterations in local environmental conditions.
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Dates et versions

hal-03862671 , version 1 (21-11-2022)

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Andrea Fanesi, Thierry Martin, Cyril Breton, Olivier Bernard, Romain Briandet, et al.. The architecture and metabolic traits of monospecific photosynthetic biofilms studied in a custom flow‐through system. Biotechnology and Bioengineering, 2022, 119, pp. 2459-2470. ⟨10.1002/bit.28147⟩. ⟨hal-03862671⟩
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