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Article Dans Une Revue International Journal of Molecular Sciences Année : 2022

Morphological and Mechanical Characterization of Extracellular Vesicles and Parent Human Synoviocytes under Physiological and Inflammatory Conditions

Samira Filali
  • Fonction : Auteur
Nesrine Darragi-Raies
  • Fonction : Auteur
Layth Ben-Trad
Agnès Piednoir
  • Fonction : Auteur
Fabrice Pirot
Ahmed Landoulsi
  • Fonction : Auteur
Agnès Girard-Egrot
Thierry Granjon
  • Fonction : Auteur
Ofelia Maniti
  • Fonction : Auteur
Pierre Miossec
  • Fonction : Auteur

Résumé

The morphology of fibroblast-like synoviocytes (FLS) issued from the synovial fluid (SF) of patients suffering from osteoarthritis (OA), rheumatoid arthritis (RA), or from healthy subjects (H), as well as the ultrastructure and mechanical properties of the FLS-secreted extracellular vesicles (EV), were analyzed by confocal microscopy, transmission electron microscopy, atomic force microscopy, and tribological tests. EV released under healthy conditions were constituted of several lipid bilayers surrounding a viscous inner core. This “gel-in” vesicular structure ensured high mechanical resistance of single vesicles and good tribological properties of the lubricant. RA, and to a lesser extent OA, synovial vesicles had altered morphology, corresponding to a “gel-out” situation with vesicles surrounded by a viscous gel, poor mechanical resistance, and poor lubricating qualities. When subjected to inflammatory conditions, healthy cells developed phenotypes similar to that of RA samples, which reinforces the importance of inflammatory processes in the loss of lubricating properties of SF.

Dates et versions

hal-03859918 , version 1 (18-11-2022)

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Citer

Samira Filali, Nesrine Darragi-Raies, Layth Ben-Trad, Agnès Piednoir, Saw-See Hong, et al.. Morphological and Mechanical Characterization of Extracellular Vesicles and Parent Human Synoviocytes under Physiological and Inflammatory Conditions. International Journal of Molecular Sciences, 2022, 23 (21), pp.13201. ⟨10.3390/ijms232113201⟩. ⟨hal-03859918⟩
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