Combining AFM, XPS and chemical hydrolysis to understand the complexity and dynamics of C. vulgaris cell wall composition and architecture - Archive ouverte HAL Access content directly
Journal Articles Algal Research - Biomass, Biofuels and Bioproducts Year : 2023

Combining AFM, XPS and chemical hydrolysis to understand the complexity and dynamics of C. vulgaris cell wall composition and architecture

Abstract

The microalgae cell wall represents its interface with its environment, and is a dynamic and complex structure that evolves depending on the growth phase or culture conditions. To apprehend this complexity, an experimental approach combining AFM, XPS, and chemical hydrolysis followed by HPAEC-PAD was developed to understand the cell wall of Chlorella vulgaris, a biotechnologicallyrelevant green microalgae species. Exponential and stationary growth stages were investigated, as well as saline stress condition inducing lipid production. Results showed that both the cell wall composition and architecture changes in stationary phase, with an increase of the lipidic fraction at the expense of the proteic fraction, changes in the polysaccharidic composition, and a significant increase of its rigidity. Under saline stress, cell wall architecture seems to be affected as importantly, its rigidity is reduced. Altogether, this study demonstrates the power of combining these three techniques to give new insights into C. vulgaris cell wall, in terms of composition and architecture, and of its dynamics in different conditions.
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Dates and versions

hal-04285172 , version 1 (18-11-2022)
hal-04285172 , version 2 (14-11-2023)

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Irem Demir-Yilmaz, Marion Schiavone, Jérôme Esvan, Pascal Guiraud, Cécile Formosa-Dague. Combining AFM, XPS and chemical hydrolysis to understand the complexity and dynamics of C. vulgaris cell wall composition and architecture. Algal Research - Biomass, Biofuels and Bioproducts, 2023, 72, pp.103102. ⟨10.1016/j.algal.2023.103102⟩. ⟨hal-04285172v2⟩
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