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Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 1998

Translation inhibitors stabilize Escherichia coli mRNAs independently of ribosome protection

Isabelle Marchand
  • Fonction : Auteur
Oleg Yarchuk
  • Fonction : Auteur
Marc Dreyfus
  • Fonction : Auteur

Résumé

Translation inhibitors such as chloramphenicol in prokaryotes or cycloheximide in eukaryotes stabilize many or most cellular mRNAs. In Escherichia coli , this stabilization is ascribed generally to the shielding of mRNAs by stalled ribosomes. To evaluate this interpretation, we examine here how inhibitors affect the stabilities of two untranslated RNAs, i.e., an engineered lacZ mRNA lacking a ribosome binding site, and a small regulatory RNA, RNAI. Whether they block elongation or initiation, all translation inhibitors tested stabilized these RNAs, indicating that stabilization does not necessarily reflect changes in packing or activity of translating ribosomes. Moreover, both the initial RNase E-dependent cleavage of RNAI and lacZ mRNA and the subsequent attack of RNAI by polynucleotide phosphorylase and poly(A)-polymerase were slowed. Among various possible mechanisms for this stabilization, we discuss in particular a passive model. When translation is blocked, rRNA synthesis is known to increase severalfold and rRNA becomes unstable. Meanwhile, the pools of RNase E and polynucleotide phosphorylase, which, in growing cells, are limited because these RNases autoregulate their own synthesis, cannot expand. The processing/degradation of newly synthesized rRNA would then titrate these RNases, causing bulk mRNA stabilization.

Dates et versions

hal-03857653 , version 1 (17-11-2022)

Identifiants

Citer

Pascal Jean Lopez, Isabelle Marchand, Oleg Yarchuk, Marc Dreyfus. Translation inhibitors stabilize Escherichia coli mRNAs independently of ribosome protection. Proceedings of the National Academy of Sciences of the United States of America, 1998, 95 (11), pp.6067-6072. ⟨10.1073/pnas.95.11.6067⟩. ⟨hal-03857653⟩
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