Three-dimensional Structure of FEZ-1, a Monomeric Subclass B3 Metallo-β-lactamase from Fluoribacter gormanii, in Native Form and in Complex with d-Captopril - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Molecular Biology Année : 2003

Three-dimensional Structure of FEZ-1, a Monomeric Subclass B3 Metallo-β-lactamase from Fluoribacter gormanii, in Native Form and in Complex with d-Captopril

I Garcı́a-Sáez
  • Fonction : Auteur
P S Mercuri
  • Fonction : Auteur
R Kahn
  • Fonction : Auteur
J M Frère
  • Fonction : Auteur
M Galleni
  • Fonction : Auteur
G M Rossolini
  • Fonction : Auteur

Résumé

The b-lactamases are involved in bacterial resistance to penicillin and related compounds. Members of the metallo-enzyme class are now found in many pathogenic bacteria and are thus becoming of major clinical importance. The structures of the Zn-b-lactamase from Fluoribacter gormanii (FEZ-1) in the native and in the complex form are reported here. FEZ-1 is a monomeric enzyme, which possesses two zinc-binding sites. These structures are discussed in comparison with those of the tetrameric L1 enzyme produced by Stenotrophomonas maltophilia. From this analysis, amino acids involved in the oligomerization of L1 are clearly identified. Despite the similarity in fold, the active site of FEZ-1 was found to be significantly different. Two residues, which were previously implicated in function, are not present in L1 or in FEZ-1. The broad-spectrum substrate profile of Zn-b-lactamases arises from the rather wide active-site cleft, where various b-lactam compounds can be accommodated.
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hal-03856347 , version 1 (16-11-2022)

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I Garcı́a-Sáez, P S Mercuri, Cyril Papamicaël, R Kahn, J M Frère, et al.. Three-dimensional Structure of FEZ-1, a Monomeric Subclass B3 Metallo-β-lactamase from Fluoribacter gormanii, in Native Form and in Complex with d-Captopril. Journal of Molecular Biology, 2003, 325, pp.651 - 660. ⟨10.1016/s0022-2836(02)01271-8⟩. ⟨hal-03856347⟩
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