Endogenous viral elements reveal associations between a non-retroviral RNA virus and symbiotic dinoflagellate genomes - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2022

Endogenous viral elements reveal associations between a non-retroviral RNA virus and symbiotic dinoflagellate genomes

Alex Veglia
  • Fonction : Auteur
Kalia S.I. Bistolas
Christian Voolstra
Benjamin Hume
  • Fonction : Auteur
Serge Planes
Denis Allemand
Emilie Boissin
Patrick Wincker
Julie Poulain
Clémentine Moulin
  • Fonction : Auteur
Guillaume Bourdin
  • Fonction : Auteur
Guillaume Iwankow
  • Fonction : Auteur
Sarah Romac
  • Fonction : Auteur
Sylvain Agostini
Bernard Banaigs
Emmanuel Boss
Chris Bowler
Colomban de Vargas
Eric Douville
  • Fonction : Auteur
Michel Flores
Didier Forcioli
Paola Furla
Eric Gilson
Fabien Lombard
Stéphane Pesant
Stéphanie Reynaud
Shinichi Sunagawa
Olivier Thomas
Romain Troublé
  • Fonction : Auteur
Didier Zoccola
Adrienne M.S. Correa
  • Fonction : Auteur
Rebecca Vega Thurber

Résumé

Abstract Endogenous viral elements (EVEs) offer insight into the evolutionary histories and hosts of contemporary viruses. This study leveraged DNA metagenomics and genomics to detect and infer the host of a non-retroviral dinoflagellate-infecting +ssRNA virus (dinoRNAV) common in coral reefs. As part of the Tara Pacific Expedition, this study surveyed 269 newly sequenced cnidarians and their resident symbiotic dinoflagellates (Symbiodiniaceae), associated metabarcodes, and publicly available metagenomes, revealing 178 dinoRNAV EVEs, predominantly among hydrocoral-dinoflagellate metagenomes. Putative associations between Symbiodiniaceae and dinoRNAV EVEs were corroborated by the characterization of dinoRNAV-like sequences in 17 of 18 scaffold-scale and one chromosome-scale dinoflagellate genome assembly, flanked by characteristically cellular sequences and in proximity to retroelements, suggesting potential mechanisms of integration. EVEs were not detected in dinoflagellate-free (aposymbiotic) cnidarian genome assemblies, including stony corals, hydrocorals, jellyfish, or seawater. The pervasive nature of dinoRNAV EVEs within dinoflagellate genomes (especially Symbiodinium ), as well as their inconsistent within-genome distribution and fragmented nature, suggest ancestral or recurrent integration of this virus with variable conservation. Broadly, these findings illustrate how +ssRNA viruses may obscure their genomes as members of nested symbioses, with implications for host evolution, exaptation, and immunity in the context of reef health and disease.

Dates et versions

hal-03854759 , version 1 (16-11-2022)

Identifiants

Citer

Alex Veglia, Kalia S.I. Bistolas, Christian Voolstra, Benjamin Hume, Serge Planes, et al.. Endogenous viral elements reveal associations between a non-retroviral RNA virus and symbiotic dinoflagellate genomes. 2022. ⟨hal-03854759⟩
24 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More